Journal: bioRxiv

Article Title: Tech Note: Simplified protocol for SMARTer Pico kit

doi: 10.1101/2025.09.02.673416

Figure Lengend Snippet:

Article Snippet: Here, Takara used human lung FFPE total RNA without fragmentation, with 16 PCR cycles for PCR2.

Techniques: Concentration Assay

Journal: Molecular Cancer Therapeutics

Article Title: OBI-992, a Novel TROP2-Targeted Antibody–Drug Conjugate, Demonstrates Antitumor Activity in Multiple Cancer Models

doi: 10.1158/1535-7163.MCT-24-0588

Figure Lengend Snippet: TROP2 expression in various cancer tissues. A, The expression levels of TROP2 in tissue samples from 19 cancer types were analyzed by IHC. Dots indicate TROP2 H -score for each sample, and solid bars indicate the median H -score of each cancer type. B, TROP2 prevalence rates ( x -axis) were calculated as the percentage of samples with TROP2 H -score ≥100. The ranking order of cancer types was based on the TROP2 prevalence rate. C, Plot of percentage of cells from tumor (blue) and corresponding normal tissues (gray) with TROP2 membrane expression determined by IHC. Each dot represents an individual sample. Solid bars indicate the median percent of TROP2-expressing cells for each tissue type. The numbers of specimens used for each cancer type are indicated in the plots. Representative IHC images of TROP2 high (3+), moderate (2+), and low (1+) expression are shown in Supplementary Fig. S3. Adeno, adenocarcinoma; BC, breast carcinoma; Ca., cancer/carcinoma; CRC, colorectal cancer; H&N, head and neck carcinoma; HER2, human epidermal growth factor receptor 2; HR, hormone receptor; HRPC, hormone-resistant prostate cancer; IHC, immunohistochemistry; NSCLC, non-small cell lung cancer; RCC, renal cell renal carcinoma; SCC, squamous carcinoma; SCLC, small cell lung carcinoma; TNBC, triple-negative breast carcinoma.

Article Snippet: Up to 448 formalin-fixed, paraffin-embedded human late-stage tumor tissue samples, representing 19 cancer types [bladder cancer, cervical adenocarcinoma, cervical squamous cell carcinoma (SCC), colorectal cancer, endometrial cancer, esophageal cancer, gallbladder cancer, gastric cancer, head and neck cancer, hormone receptor-positive (HR + ) breast cancer, human epidermal growth factor receptor 2-positive (HER2 + ) breast cancer, non-small cell lung cancer (NSCLC) adenocarcinoma, NSCLC SCC, ovarian adenocarcinoma, pancreatic cancer, prostate cancer, renal cell carcinoma (RCC), small cell lung cancer (SCLC), and triple-negative breast cancer (TNBC)], were purchased from AMS Biotechnology.

Techniques: Expressing, Membrane, Immunohistochemistry

Journal: Molecular Cancer Therapeutics

Article Title: OBI-992, a Novel TROP2-Targeted Antibody–Drug Conjugate, Demonstrates Antitumor Activity in Multiple Cancer Models

doi: 10.1158/1535-7163.MCT-24-0588

Figure Lengend Snippet: TROP2 expression in various cancer tissues. A, The expression levels of TROP2 in tissue samples from 19 cancer types were analyzed by IHC. Dots indicate TROP2 H -score for each sample, and solid bars indicate the median H -score of each cancer type. B, TROP2 prevalence rates ( x -axis) were calculated as the percentage of samples with TROP2 H -score ≥100. The ranking order of cancer types was based on the TROP2 prevalence rate. C, Plot of percentage of cells from tumor (blue) and corresponding normal tissues (gray) with TROP2 membrane expression determined by IHC. Each dot represents an individual sample. Solid bars indicate the median percent of TROP2-expressing cells for each tissue type. The numbers of specimens used for each cancer type are indicated in the plots. Representative IHC images of TROP2 high (3+), moderate (2+), and low (1+) expression are shown in Supplementary Fig. S3. Adeno, adenocarcinoma; BC, breast carcinoma; Ca., cancer/carcinoma; CRC, colorectal cancer; H&N, head and neck carcinoma; HER2, human epidermal growth factor receptor 2; HR, hormone receptor; HRPC, hormone-resistant prostate cancer; IHC, immunohistochemistry; NSCLC, non-small cell lung cancer; RCC, renal cell renal carcinoma; SCC, squamous carcinoma; SCLC, small cell lung carcinoma; TNBC, triple-negative breast carcinoma.

Article Snippet: Up to 448 formalin-fixed, paraffin-embedded human late-stage tumor tissue samples, representing 19 cancer types [bladder cancer, cervical adenocarcinoma, cervical squamous cell carcinoma (SCC), colorectal cancer, endometrial cancer, esophageal cancer, gallbladder cancer, gastric cancer, head and neck cancer, hormone receptor-positive (HR + ) breast cancer, human epidermal growth factor receptor 2-positive (HER2 + ) breast cancer, non-small cell lung cancer (NSCLC) adenocarcinoma, NSCLC SCC, ovarian adenocarcinoma, pancreatic cancer, prostate cancer, renal cell carcinoma (RCC), small cell lung cancer (SCLC), and triple-negative breast cancer (TNBC)], were purchased from AMS Biotechnology.

Techniques: Expressing, Membrane, Immunohistochemistry